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Protein Engineering

(Modulnummer MT.2.209)

Häufigkeitjedes Studienjahr
Dauer2 Semester
ECTS Credits6

Verwendbarkeit des Modules



Practical course Protein-Engineering:

All experiments were developed and performed under involvement of students in cooperation with the Institute for Molecular Cell Biology (Director Prof. Dr. R. Wetzker) and partially with the pharmaceutical company Serono (Geneve, Switzerland) and the Oncological Research Laboratory of the Friedrich-Schiller-University (head Dr. J. Clement).
Using a novel therapeutic targeting protein, PI3Kinase, the full potential of protein engineering will be demonstrated for pharmaceutical application. To illustrate this recent development all students will get a copy of the paper: R. Wetzker und C. Rommel. Phosphoinositide 3-kinase as targets for therapeutic intervention. Current Pharmaceutical Design 10, 1915-1922 (2004).
For further theoretical and practical support of the practical master courses contracts are planned between the Forschungszentrum für Molekulare Biomedizin and the Onkologisches Forschungslabor am Klinikum der FSU. They will enable the constant offer of the highest possible level in future education in this field of biotechnology.

Practical course:


Students learn about the potential of protein design and construction in biotechnology and pharmaceutical application. The main focus will be novel development of protein products and diagnostics. Profound knowledge about protein structure will be communicated as prerequisite for all protein engineering work. The unlimited potential of protein engineering will be demonstrated theoretically and also as planning and established work protocols: Targeting via exchange, deletion, insertion and fusion of one or any number of amino acid residues resp. peptides for all therapeutic and diagnostic applications. Special methods like PCR without primers etc. will be communicated.
The practical course is the continuation and necessary completion of the laboratory course Genetic Engineering and Molecular Cell Biology. It is designed as integrated practical course with that of Molecular Cell Biology. It will respond to the rapidly growing demand for graduates especially trained in this important field of biotechnology. The Students experience the direct correlation between the current developments of protein engineering with functional analysis in higher cells: Identification of novel targets, drug design, development of molecular tools for diagnostics and therapy. Biocomputing will be performed concerning 3D-imaging of protein molecules, protein-protein-interaction, labelling of selected targeting-amino acid residues, sequence-based analysis of secondary and tertiary structures, homology-modelling and alignment-analyses.

Lehr- und Lernformen

Teil 1Teil 2
Vorlesung (SWS)11
Übung (SWS)10
Praktikum (SWS)02
gesamt (SWS)23


Kenntnisse der Molekularen Zellbiologie.

Voraussetzungen für die Vergabe von ECTS Credits

Schriftliche Prüfungsleistung (90 min.), Laborschein

Arbeitsaufwand (workload)

Präsenzstunden (h)75
Selbststudium (h)105
Gesamtzeitaufwand (h)180


Skript und Praktikumsanleitung im Intranet als pdf-Datei


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